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BCH Order Code FER Semen Analysis, Semen

Reporting Name

Semen Analysis

Useful For

Determining male fertility status

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Ordering Guidance

This test should not be used to check patient's sterility following a vasectomy. For such cases, order POSV / Post Vasectomy Check, Semen.


Semen analysis specimens submitted to Mayo Clinic Laboratories are not acceptable for fructose testing due to the use of dilution media. For specimen requirements for fructose testing in azoospermia patients, see FROS2 / Fructose, Qualitative, Semen.


Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.

Shipping Instructions

Specimen must arrive within 24 hours of collection. Send specimen Monday through Thursday only and not the day before a holiday. If holiday falls on a Saturday, holiday will be observed on the preceding Friday. Sunday holidays are observed on the following Monday. Specimen should be collected and packaged as close to shipping time as possible. Laboratory does not perform testing on weekends.

Necessary Information

Include the following information:

-Semen volume (required)



-Appearance (color)

-Number of days of sexual abstinence

Specimen Required

Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.

Supplies: Semen Analysis Kit - Dilution Media (T178)

Specimen Volume: Total ejaculate

Collection Instructions:

1. After collection, allow the specimen to liquefy for 1 hour.

2. Measure the volume. If necessary, also measure appearance, pH, and viscosity.

3. Place the specimen into media within 1 hour.

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Semen Ambient

Reference Values

Appearance: normal

Volume: ≥1.5 mL

pH: ≥7.2

Motile/mL: ≥6.0 x 10(6)

Sperm/mL: ≥15.0 x 10(6)

Motility: ≥40%

Grade: ≥2.5

Note: Multiple laboratory studies have indicated that semen parameters for motility and grade on average retain 80% of original parameters when our shipping method is used for transport. Using these averages, samples with 32% to 39% motility and grade of 2 may be in the normal range if testing was performed shortly after collection. Therefore, these borderline patients may need to collect another sample at a local fertility center to verify fertility status.

Motile/ejaculate: ≥9.0 x 10(6)

Viscosity: ≥3.0

Agglutination: ≥3.0

Supravital: ≥58% live

Fructose: positive

Note: Fructose testing cannot be performed on semen analysis specimens shipped through Mayo Clinic Laboratories.

Submit separate specimen to rule out ejaculatory duct blockage. Positive result indicates no blockage.

Day(s) Performed

Monday through Friday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
FER Semen Analysis 54231-6


Result ID Test Result Name Result LOINC Value
ABSTN Abstinence 10587-4
CLST1 Collection Site 56816-2
TY Study Type 54453-6
CNTN Container Type 74384-9
APP3 Appearance 13359-5
VL53 Semen Volume 3160-9
PH1 pH 2752-4
MOTML Motile/mL 42531-4
SPML Sperm/mL 9780-8
MOTY Motility 6800-7
GR2 Grade 13942-8
MOTEJ Motile/Ejaculate 6800-7
VISC Viscosity 32789-0
AGGLU Agglutination 33217-1
STAIN Supravital Stain 4466-9
FRCT Fructose 13943-6
CMT45 Comment 48767-8

Report Available

1 to 4 days

Method Name