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HelpBCH Order Code TLYM T-Cell Lymphoma, FISH, Tissue
Ordering Guidance
This assay detects chromosome abnormalities observed in paraffin-embedded tissue samples of patients with T-cell lymphoma. For testing the blood and bone marrow from patients with T-cell lymphoma, see TLPF / T-Cell Lymphoma, FISH, Blood or Bone Marrow.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Lymph node
Preferred: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.
Acceptable: Slides
Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.
Specimen Type: Solid tumor
Preferred: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.
Acceptable: Slides
Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas
Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphomas
Providing potentially prognostic information in patients with documented systemic anaplastic lymphoma kinase-negative anaplastic large cell lymphoma
Supporting the diagnosis of peripheral T-cell lymphoma when coordinated with consultation by anatomic pathology
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _PRAH | Probe, Each Additional (TLYM) | No, (Bill Only) | No |
Testing Algorithm
This test does not include a pathology consultation. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.
A charge and CPT code is applied for each probe set hybridized, analyzed, and reported.
Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the Clinical Information.
If the patient is being tracked for known abnormalities, indicate which probes should be used.
Panel includes testing for the following abnormalities using the probes listed:
14q32.1 rearrangement, TCL1A
14q11.2 rearrangement, TRAD
-7/7q-/i(7q), D7S486/D7Z1
+8, D8Z2/MYC
2p23.2 rearrangement, ALK
3q28 rearrangement, TP63 and TBL1XR1/TP63 (only performed when necessary to resolve or confirm TP63 rearrangement concerns)
6p25.3 rearrangement, IRF4 (DUSP22)
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
T-cell Lymphoma, FISH, TissueSpecimen Type
TissueSpecimen Minimum Volume
For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides. Include 1 hematoxylin and eosin-stained slide.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Tissue | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88377-if 1 probe set
88377 x 2-if 2 probe sets
88377 x 3-if 3 probe sets
88377 x 4-if 4 probe sets
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TLYM | T-cell Lymphoma, FISH, Tissue | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 603140 | Result Summary | 50397-9 |
| 603141 | Interpretation | 69965-2 |
| 603142 | Result Table | 93356-4 |
| 603143 | Result | 62356-1 |
| GC040 | Reason for Referral | 42349-1 |
| 603144 | Specimen | 31208-2 |
| 603145 | Source | 31208-2 |
| 603146 | Tissue ID | 80398-1 |
| 603147 | Method | 85069-3 |
| 603148 | Additional Information | 48767-8 |
| 603149 | Disclaimer | 62364-5 |
| 603150 | Released By | 18771-6 |
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.